Coding
RFP-AB-Bsa
Part:BBa_K349001:Experience
Designed by: David Lloyd Group: iGEM10_Alberta (2010-10-20)
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Applications of BBa_K349001
Digestion with BsaI around the recognition sites inside of the prefix and suffix will create an RFP cassette with an A overhang at the 5' end and a B overhang at the 3' end. These ends are consistent with the BioBytes 2.0 assembly method. This is a base plasmid, meaning that the RFP cassette could be digested out with BsaI and replaced with a PCR product that has had BsaI recognition sites and the appropriate AB ends incorporated into it. This allows for the production of more novel AB Bytes.
User Reviews
UNIQ893c330ee8bed3e8-partinfo-00000000-QINU UNIQ893c330ee8bed3e8-partinfo-00000001-QINU